Competencies | Neuronax

- Feasibility study: study design, scientific and technical analysis

- Specification definition: report finalization and writing

In vitro and preclinical study design:

- choice of study model

- determination and selection of tests answering your issue

- planning and budget draw up

- development of customized in vitro protocols

- Cell maintenance and production: human or animal primary cells and cell lines

- Cell transfection

- Nervous cell culture: primary culture, in vitro selection, characterization

- Bone marrow stem cell culture: production of mononuclear cells, in vitro selection, characterization

Protocol development and optimization:

- for research or clinical use

- cell expansion protocols

- cell differentiation protocols (neurologic,osteogenic…)

- development of potency assay

In vitro tests:

- cell viability

- proliferation measurement

- differentiation studies (neural, osteoblastic…)

Biochemical analysis:

- immunophenotype

- factor secretion

- protein localization and quantification

Molecular analysis:

- RNA and DNA extraction

- quantitative RT-PCR

- gene expression profiling on chip

Study design:

- scientific and technical feasability study

- specification definition

- choice of cellular material and/or customized in vitro conditions (compound concentration, cell density)

Toxic effects on human or animal cells

- cell viability and proliferation

- cell death

- customized model development

Effects on human or animal nervous cells:

- neurotrophic and neuroprotective effects

- neurite number, length and ramification measurement

- neural protein detection